GSM1862264: WRRh; Gallus gallus; RNA-Seq - SRA

SRX1165467: GSM1862264: WRRh; Gallus gallus; RNA-Seq
8 ILLUMINA (Illumina HiSeq 2000) runs: 28.3M spots, 5.7G bases, 3.6Gb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: A Genome-wide mRNA Screen and Functional Analysis Reveal FOXO3 as a Candidate Gene for Chicken Growth

PRJNA294010 • SRP062880 • All experiments • All runs

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The objective of this study was to identify candidate genes associated with chicken growth and investigate their potential mechanisms. We used RNA-Seq to study the breast muscle transcriptome in high and low tails of Recessive White Rock (WRRh, WRRl) and Xinghua chickens (XHh, XHl). A total of 60, 23, 153 and 359 differentially expressed genes were detected in WRRh vs. WRRl, XHh vs. XHl, WRRh vs. XHh and WRRl vs. XHl, respectively. GO, KEGG pathway and gene network analyses showed that CEBPB, FBXO32, FOXO3 and MYOD1 played key roles in growth. The functions of FBXO32 and FOXO3 were validated. Overall design: Examination of mRNA profiles in two-tail samples of WRR and XH strains.

Sample: WRRh

SAMN04013381 • SRS1045977 • All experiments • All runs

Organism: Gallus gallus

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was extracted from each breast muscle using Trizol (Invitrogen, CA, USA) according to manufacturer’s instructions. The quality and concentration of all twelve RNA samples were determined by 1.5% agarose gel electrophoresis and absorbance at A260/280 ratio, and then divided to four mixed RNA pools for WRRh, WRRl, XHh, and XHl. mRNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM1862264

Links:

NCBI link: NCBI Entrez (gds)

Runs: 8 runs, 28.3M spots, 5.7G bases, 3.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR2185589	4,000,000	808M	527.6Mb	2015-08-28
SRR2185590	4,000,000	808M	528.3Mb	2015-08-28
SRR2185591	4,000,000	808M	524.8Mb	2015-08-28
SRR2185592	4,000,000	808M	525.5Mb	2015-08-28
SRR2185593	4,000,000	808M	532.5Mb	2015-08-28
SRR2185594	4,000,000	808M	521.5Mb	2015-08-28
SRR2185595	4,000,000	808M	522.9Mb	2015-08-28
SRR2185596	335,362	67.7M	44.5Mb	2015-08-28

ID:: 1695587

SRA

Sequence Read Archive

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